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Sanger Sequencing

A.  Individual Samples (<24 samples)

    • Use a 1.5 mL microfuge tube
    • Provide at least 10 uL of sample (12-15 uL preferred)
    • Centrifuge your sample before dropping it off at DSF
    • Write your order number and sample NUMBER legibly on the cap             

B.  96 Well Plate (>24 samples)

    • Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
    • Provide at least 10 uL of sample (12-15 uL preferred)
    • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
    • Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
    • Write your order number legibly on the plate and foil
    • Put your samples in order vertically, starting with well A1
          

PCR Cleanup

 A.  Individual Samples (<24 samples)

    • Use a 1.5 mL microfuge tube
    • Provide at least 20 uL of unpurified PCR reaction
    • Centrifuge your sample before dropping it off at DSF
    • Write your order number and sample NUMBER legibly on the cap
    • Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
    • Primer should be provided at 10 uM concentration  
    • Write the order number AND primer name legibly on the cap.                                      

B.  96 Well Plate (>24 samples)

    • Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
    • Provide at least 20 uL of unpurified PCR reaction
    • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
    • Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
    • Write your order number legibly on the plate and foil
    • Put your samples in order vertically, starting with well A1
    • Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
    • Primer should be provided at 10 uM concentration  
    • Write the order number AND primer name legibly on the cap

                                                

 

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