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samtools mpileup -uf samtools_bowtie/REL606.5.fasta samtools_bowtie/sorted_REL606.5.bam \\|bcftools view -vcg - \> samtools_bowtie/output.vcf
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title

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Exercise.

VCF format has Allele Frequency tags denoted by AF1. Try the following command to see what value we have in our files.

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