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What does the -v flag do in grep?

grep -v *something*

cat input.vcf | grep AF1=1 > output.vcf

Is not practical, since we will lose vital VCF formatting and may not be able to use this file in the future.

cat input.vcf | grep -v AF1=0 > output.vcf

Will preserve all lines that don't have a AF1=0 value and is one way of doing this.

sed -i '/AF1=0/ d' input.vcf

Is a way of doing it in-line and not requiring you to make another file.