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Next we edit the multiqc_config.yaml configuration file to add appropriate custom data sections:x

Code Block
titlemultiqc_config.yaml
# Titles to use for the report.
title: "ATAC-Seq QC Reports"
subtitle: null
intro_text: "MultiQC reports for Igor's ATAC-Seq proof-of-concept project."
report_header_info:
    - Sequenced by: 'GSAF'
    - Job: 'JA17277'
    - Run: 'SA17121'
    - Setup: '2x150'

# Change the output filenames
output_fn_name: mqc_report.html
data_dir_name: mqc_report_data

# Ignore these files / directories / paths when searching for reports
fn_ignore_files:
    - '*.dupinfo.txt'

# Modules that should come at the top of the report
top_modules:
    - 'generalstats'
    - 'fastqc'
    - 'samtools'
    - 'picard'

# --------------------------------
# Custom data
# --------------------------------

custom_data:
    bowtie2_isize:
        id: 'bowtie2_isize_section'
        section_name: 'Bowtie2 insert size'
        description: 'distribution for alignments (bowtie2 --local -X2000 --no-mixed --no-discordant)'
        file_format: 'tsv'
        plot_type: 'linegraph'
        pconfig:
            id: 'bowtie2_isize_plot'
            title: 'Insert sizes for proper pairs'
            xlab: 'Insert size'
            ylab: 'Count'
sp:
    bowtie2_isize_section:
        fn: '*.bowtie2_isizes.tsv'
Expand
titleCatch up

To catch up, just use Anna's pre-made files:

Code Block
languagebash
mkdir -p $SCRATCH/byteclub/multiqc
cd $SCRATCH/byteclub/multiqc
rsync -avrP --delete /work/projects/BioITeam/projects/byteclub/multiqc/06_custom_linegraph/ .

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