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  • -q N – only report alignment records with mapping quality of at least N (>= N).
  • -f 0xXX – only report alignment records where the specified flags are all set (are all 1)
    • you can provide the flags in decimal, or as here as hexadecimal
  • -F 0xXX – only report alignment records where the specified flags are all cleared (are all 0)

Setup

Login to ls6.tacc.utexas.edu and start an idev session.

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