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Unzip MWU.zip on personal computer.

Has The MWU directory three subdirectories:

exercise1_fisher_test

...

exercise3_kog_mwu

 

  1. Example enrichment test analysis using Fisher's Exact test
    1. open exercise1_fisher_test
    2. open the R script 'example_fisher_test.R'
    3. set the working directory as the path to 'exercise1_fisher_test/'
    4. execute the commands in the script to see if the fake GO term 'schmooase' is enriched among the differentially expressed genes.
  2. GO Mann-Whitney U Enrichment test
    1. open exercise2_go_mwu
    2. open the R script 'GO_MWU.R'
    3. set working directory as the path to 'exercise2_go_mwu/'
    4. execute the commands to build a dendrogram of enriched Cellular Component GO terms
    5. Edit the script to assign the goDivision variable to look at Molecular Function instead of Cellular Component
    6. Re-execute all the commands to build the dendrogram for Molecular Function GO terms
  3. kog Mann-Whitney U Enrichment analysis
    1. open KOG_MWU_heat.R
    2. set working directory
    3. use bioconductor to install the package
    4. execute the commands
    5. What do the results suggest about transcriptional response to heat stress in adult and larval corals?