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Local file systems

There are 3 local file systems available on any TACC compute cluster (Lonestar5stampede2, stampede2 lonestar5, etc.), each with different characteristics. All these local file systems are very fast and set up for parallel I/O (Lustre file system).

On ls5 stampede2 these local file systems have the following characteristics:

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--------------------- Project balances for user abattenh ----------------------
| Name           Avail SUs     Expires | Name           Avail SUs     Expires |
| genomeAnalysis       673  2021-03-31 | BioinformaticsResour 100  2020-06-30 |
| UT-2015-05-18       1000  2021-03-31 | DNAdenovo           4969  2021-03-31 |
| CancerGenetics      4856  2020-09-30 | A-cm10              8867  2020-12-31 |
------------------------ Disk quotas for user abattenh ------------------------
| Disk         Usage (GB)     Limit    %Used   File Usage       Limit   %Used |
| /home1              0.0      10.0     0.10          153     1000000    0.02 |
| /workwork2             614.5    1024.0    60.01        61094     3000000    2.04 |
| /scratch         2676.6       0.0     0.00        32442           0    0.00 |
-------------------------------------------------------------------------------

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When you first login, you start in your home directory. Use these the cd, cdw2 and cds commands to change to your other file systems. Notice how your command prompt helpfully changes to show your location.

cdwcdw2
cds
cd

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TACC compute clusters now share a common Work file system called stockyard. So files in your Work area do not have to be copied, for example from ls5 to stampede2 to ls5 – they can be accessed directly from either cluster.

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• $STOCKYARD - This refers to the root of your shared Work area
  • e.g. /work/01063/abattenh  (should be changed to /work2/01063/abattenh soon)
    
• $WORK or $WORK2 - Refers to a sub-directory of the shared Work area that is different for different clusters, e.g.:
  
  • /work/01063/abattenh/lonestar on ls5
  • /workwork2/01063/abattenh/stampede2 on stampede2

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ls /workwork2/projects/BioITeam

Files we will use in this course are in a sub-directory there. The $CORENGS environment variable set in your login profile refers to this path.

echo $CORENGS
ls /workwork2/projects/BioITeam/projects/courses/Core_NGS_Tools

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corral is a gigantic (multiple PB) storage system (spinning disk) where researchers can store data. UT researchers may request up to 5 TB of corral storage through the normal TACC allocation request process. Additional space on corral can be rented for ~$85~$80/TB/year.

The UT/Austin BioInformatics Team also has an older, common directory area on corral.

ls /corral-repl/utexas/BioITeam

A couple of things to keep A couple of things to keep in mind regarding corral:

• corral is a great place to store data in between analyses.
  • Store your permanent, original sequence data on corral
  • Copy the data you want to work with from corral to $SCRATCH
  • Run your analyses (batch jobs)
  • Copy your results back to corral
• Occasionally corral can become unavailable. This can cause any command to hang that tries to access corral data!

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Once that data is staged to the ranch disk it can be copied to other places. However, the ranch file system is not mounted as a local file system from the stampede2 or ls5 clusters. So remote copy commands are always needed to copy data to and from ranch (e.g. scp, rsync).

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• original – for original sequencing data (compressed FASTQ files)
  • sub-directories named, for example, by year_month.<project_name>
• aligned – for alignment artifacts (BAM files, etc)
  • sub-directories named, e.g.,  by year_month.<project_name>
• analysis – further downstream analysis
  • reasonably named sub-directories, often by project
• genome refs – reference genomes and other annotation files used in alignment and analysis
  
  • sub-directories for different reference genomes
  • e.g. ucsc/hg19, ucsc/sacCer3, mirbase/v20
• code – for scripts and programs you and others in your organization write
  • ideally maintained in a version control system such as git, subversion or cvs.
  • easiest to name sub-directories for people.

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mkdir -p $WORK$SCRATCH/archive/original/20202021.core_ngs
cd $WORK$SCRATCH/archive/original/20202021.core_ngs
wget 

Here are two web links:

• httphttps://web.corral.tacc.utexas.edu/BioITeamBioinformaticsResource/CoreNGS/yeast_stuff/Sample_Yeast_L005_R1.cat.fastq.gzhttp
• https://web.corral.tacc.utexas.edu/BioinformaticsResource/BioITeamCoreNGS/yeast_stuff/Sample_Yeast_L005_R2.cat.fastq.gz

Right-click (Windows) or Control+click (Mac) on the 1st link in your browser, then select "Copy link location" from the menu. Now go back to your Terminal. Put your cursor after the space following the wget command then either right-click (Windows), or Paste (Command-V on Mac, Control-V on Windows). The command line to be executed should now look like this:

wget http://web.corral.tacc.utexas.edu/BioITeamBioinformaticsResource/CoreNGS/yeast_stuff/Sample_Yeast_L005_R1.cat.fastq.gz

Now press Enter to get the command going. Repeat for the 2nd link. Check that you now see the two files (ls).

Copy from a corral location - cp or rsync

Suppose you have a corral allocation or stockyard area where your organization keeps its data, and that the sequencing data has been downloaded there. You can use various Linux commands to copy the data locally from there to your $SCRATCH area.

cp

The cp command copies one or more files from a local source to a local destination. It has the most common form:

cp [options] <source file 1> <source file 2> ... <destination directory>/

Make a directory in your scratch area and copy a single file to it. The trailing slash ( / ) on the destination says it is a directory.

mkdir -p $SCRATCH/data/test1
cp $CORENGS/misc/small.fq  $SCRATCH/data/test1/
ls $SCRATCH/data/test1

# or..
mkdir -p ~/scratch/data/test1
cd ~/scratch/data/test1
cp $CORENGS/misc/small.fq  .
ls

Copy from a corral location - cp or rsync

Suppose you have a corral allocation or stockyard area where your organization keeps its data, and that the sequencing data has been downloaded there. You can use various Linux commands to copy the data locally from there to your $SCRATCH area.

cp

The cp command copies one or more files from a local source to a local destination. It has the most common form:

cp [options] <source file 1> <source file 2> ... <destination directory>/

Make a directory in your Scratcharea and copy a single file to it. The trailing slash ( / ) on the destination says the destination is a directoryCopy an entire directory to your scratch area. The -r argument says "recursive".

mkdir -p $SCRATCH/data/test1
cds
cd data
cp -r $CORENGS/general/ general/

Exercise: What files were copied over?

ls general

BEDTools-User-Manual.v4.pdf  SAM1.pdf  SAM1.v1.4.pdf

local rsync

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rsync is a very complicated program, with many options (http://rsync.samba.org/ftp/rsync/rsync.html). However, if you use the recipe shown here for directories, it's hard to go wrong:

rsync -avW local/path/to/source_directory/ local/path/to/destination_directory/

Both the source and target directories are local (in some file system accessible directly from ls5). Either full or relative path syntax can be used for both. The -avP options above stand for:

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• -p – preserve file permissions
• -t – preserve file times
• -l – copy symbolic links as links
• -r – recursively copy sub-directories

cp $CORENGS/misc/small.fq  $SCRATCH/data/test1/
ls $SCRATCH/data/test1

# or..
mkdir -p ~/scratch/data/test1
cd ~/scratch/data/test1
cp $CORENGS/misc/small.fq  .
ls

Copy an entire directory to your Scratch area. The -r option says "recursive".

mkdir -p $SCRATCH/data
cds
cd data
cp -r $CORENGS/general/ general/

Exercise: What files were copied over?

ls general

BEDTools-User-Manual.v4.pdf  SAM1.pdf  SAM1.v1.4.pdf

local rsync

The rsync command is typically used to copy whole directories. What's great about rsync is that itonly copies what has changed in the source directory. So if you regularly rsync a large directory to TACC, it may take a long time the 1st time, but the 2nd time (say after downloading more sequencing data to the source), only the new files will be copied.

rsync is a very complicated program, with many options (http://rsync.samba.org/ftp/rsync/rsync.html). However, if you use the recipe shown here for directories, it's hard to go wrong:

rsync -avW local/path/to/source_directory/ local/path/to/destination_directory/

Both the source and target directories are local (in some file system accessible directly from stampede2). Either full or relative path syntax can be used for both. The -avW options above stand for:

• -a means "archive mode", which implies the following options (and a few others)
  
  • -p – preserve file permissions
  • -t – preserve file times
  • -l – copy symbolic links as links
  • -r – recursively copy sub-directories
• -v means verbose
• -W means transfer Whole file only
  • Normally the rsync algorithm compares the contents of files that need to be copied and only transfers the different parts.
  • For large files and binary files, figuring out what has changed (diff-ing) can take more time than just copying the whole file.
  • The -W option disables file content comparisons (skips diff-ing).

Since these are all single-character options, they can be combined after one option prefix dash ( - ). You could also use options -ptlrvW, separately, instead of using -a for "archive mode".

mkdir -p $SCRATCH/data
cds
rsync -avW $CORENGS/custom_tracks/ data/custom_tracks/

Exercise: What files were copied over?

ls $SCRATCH/data/custom_tracks
# or
ls ~/scratch/data/custom_tracks
# or
cds; cd data/custom_tracks; ls

Now repeat the rsync and see the difference.

Use the Up arrow to retrieve the previous command from your bash command history.

rsync -avW /work/projects/BioITeam/projects/courses/Core_NGS_Tools/custom_tracks/ data/custom_tracks/

Copy from a remote computer - scp or rsync

Provided that the remote computer is running Linux and you have ssh access to it, you can use various Linux commands to copy data over a secure connection.

The good news is that once you have learned cp and local rsync, remote secure copy (scp) and remote rsync are very similar!

scp

The scp command copies one or more files from a source to a destination, where either source or destination, or both, can be a remote path.

Remote paths are similar to local paths, but have user and host information first:

user_name@full.host.name:/full/path/to/directory/or/file

– or –

user_name@full.host.name:~/path/relative/to/home/directory

Copy a single file to your $SCRATCH/data/test1 directory from the server named dragonfly.icmb.utexas.edu, using the user account corengstools. When prompted for a password, use the one we have written to the Zoom chat (or copy/paste the password from this file: $CORENGS/tacc/dragonfly_access.txt)

cat $CORENGS/tacc/dragonfly_access.txt
cds
mkdir -p data/test2
scp corengstools@dragonfly.icmb.utexas.edu:~/custom_tracks/progeria_ctcf.vcf.gz ./data/test2/
ls ./data/test2

Notes:

• The 1st time you access a new host the SSH security prompt will appear
• You will be prompted for your remote host password
• The  -r recursive argument works for scp also, just like for cp

remote rsync

rsync can be run just like before, but using the remote-host syntax. Here we use two tricks:

• The tilde ( ~ ) at the start of the path means "relative to my home directory"
• We use the tilde ( ~ ) in the destination to traverse the scratch symbolic link in your home directory.
  
  

rsync -avW corengstools@dragonfly

...

• Normally the rsync algorithm compares the contents of files that need to be copied and only transfers the different parts.
• For large files and binary files, figuring out what has changed (diff-ing) can take more time than just copying the whole file.
• The -W option disables file content comparisons (skips diff-ing).

Since these are all single-character options, they can be combined after one option prefix dash ( - ). You could also use options -ptlrvW, separately, instead of using -a for "archive mode".

mkdir -p $SCRATCH/data
cds
rsync -avWP $CORENGS/custom_tracks/ data/custom_tracks/

Exercise: What files were copied over?

ls $SCRATCH/data/custom_tracks
# or
ls ~/scratch/data/custom_tracks
# or
cds; cd data/custom_tracks; ls

Now repeat the rsync and see the difference.

Use the Up arrow to retrieve the previous command from your bash command history.

rsync -avWP /work/projects/BioITeam/projects/courses/Core_NGS_Tools/custom_tracks/ data/custom_tracks/

Copy from a remote computer - scp or rsync

Provided that the remote computer is running Linux and you have ssh access to it, you can use various Linux commands to copy data over a secure connection.

The good news is that once you have learned cp and local rsync, remote secure copy (scp) and remote rsync are very similar!

scp

The scp command copies one or more files from a source to a destination, where either source or destination, or both, can be a remote path.

Remote paths are similar to local paths, but have user and host information first:

user_name@full.host.name:/full/path/to/directory/or/file

– or –

user_name@full.host.name:~/path/relative/to/home/directory

Copy a single file to your $SCRATCH/data/test1 directory from the server named gapdh.icmb.utexas.edu, using the user account corengstools. When prompted for a password, use the one we have written on the board (or copy/paste the password from this file: $CORENGS/tacc/gapdh_access.txt)

cds
cat $CORENGS/tacc/gapdh_access.txt
scp corengstools@gapdh.icmb.utexas.edu:~/custom_tracks/progeria_ctcf.vcf.gz . ~/scratch/data/test1/
ls ./data/test1

Notes:

• The 1st time you access a new host the SSH security prompt will appear
• You will be prompted for your remote host password
• The  -r recursive argument works for scp also, just like for cp

remote rsync

rsync can be run just like before, but using the remote-host syntax. Here we use two tricks:

• The tilde ( ~ ) at the start of the path means "relative to my home directory"
• We traverse through the BioITeam symbolic link created in your home directory earlier.
• We use the same tilde ( ~ ) in the destination to traverse the scratch symbolic link in your home directory.

Don't forget to change userid below.

rsync -ptlrvP corengstools@gapdh.icmb.utexas.edu:~/custom_tracks/ ~/scratch/data/custom_tracks/

Exercise: Was anything copied?

Scavenger hunt exercise

Here's a fun scavenger hunt for more practice. Issue the following commands to get practice what you've learned so far:

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custom_tracks/

Exercise: Was anything copied?

Scavenger hunt exercise

Here's a fun scavenger hunt for more practice. Issue the following commands to get practice what you've learned so far:

To get started:

cd
cp -r /work2/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/what what
# or using the $CORENGS environment variable
cp -r $CORENGS/linuxpractice/what what
cd what
cat readme

Where are you when you're all done?

step by step answers

mkdir starts
cd starts
cp /work2/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/steps/nextInstr .
cat nextInst

mkdir here
cd 

here

Where are you when you're all done?

step by step answers

wget http://web.corral.tacc.utexas.edu/BioinformaticsResource/CoreNGS/step3.txt
cat step3.txt

mkdir starts
cd starts
cp /workscp -r /work2/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/changes/ changes/
# or
rsync -ptrvP /work2/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/stepschanges/nextInstr .changes/
cat nextInst

mkdir here
cd here
wget http://web.corral.tacc.utexas.edu/BioITeam/step3.txt
cat step3.txt

rsync -avP /work/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/changes/ changes/
# or
scp -r scp -r /work/projects/BioITeam/projects/courses/Core_NGS_Tools/linuxpractice/changes changes
# Note: rsync -avP ... will also work, but will report an error because the destination file and
# directory ownership cannot be changed to match the source. But the files will be copied, and
# ownership assigned to you.
 
# Then
cd changes 
more largeFile.txt

rsync -avP corengstools@gapdh corengstools@dragonfly.icmb.utexas.edu:~/the/ the/
# or
scp -r corengstools@dragonfly.icmb.utexas.edu:~/the/ the/

cd the
cat instr5.txt
cd world
cat instr6.txt

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