...
- If you want to identify novel transcripts in your particular samples
- If you want to look for differential expression in these novel transcripts
Part 3. Tag-Seq
Tag-See is targeted sequencing of just the 3' ends of mRNA.
- This is done at GSAF using a template switching protocol.
- It offers a reduction in library prep and sequencing cost.
- Consider it when looking to do differential expression analysis of known genes with more replicates, conditions, timepoints etc.
- Analysis involves some extra preprocessing. Other steps are similar to standard RNA-Seq analysis.
BACK TO COURSE OUTLINE