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Our equipment and capabilities include:
- Two One Illumina HiSeq 4000 and two HiSeq 2500 next-generation DNA sequencers and associated peripherals. The HiSeq 4000 can generate over 600 gigabases 1 Terabases of sequence in an 116-day run or 120 gigabases in a ~1 day rapid run. The GSAF has experience generating small RNA, mRNA, genomic DNA fragment, RAD (including ddRAD), bacterial and fungal metagenomics, and genomic DNA large-insert mate-pair libraries for the Illumina platform.
- Two Illumina MiSeq next-generation DNA sequencers and associated peripherals. The MiSeq is intended for lower data output, faster turn-around time projects, or for projects requiring longer read lengths (up to 600 bp per template, as two 300 bp sequences).
- Two Illumina NextSeq 500 next-generation DNA sequencer and associated peripherals. The NextSeq is ideal for intermediate scale projects, requiring more read depth than is feasible on the MiSeq platform but with faster turn-around time than the HiSeq platform.
- Informatic tools and hardware sufficient for analysis of next-generation DNA sequencing data, including:
- Multiple Dell PowerEdge R900 & R720 servers with 16-32 cores each, with BWA, Bowtie, Shrimp, SOAP, and mapreads aligners, NCBI BLAST, and R/Bioconductor, and several de novo assemblers, sequence editors, and genome browsers.
- The resources of Lonestar, a 2230,656 000 core supercomputer, and Stampede, a 400,000 core supercomputer, both hosted by UT’s Texas Advanced Computing Center, with 15 petabyte of scratch disk storage.
- The GSAF has software stacks and experience appropriate to your project and both we and TACC staff can assist you in training your students to use this resource effectively.
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Instruments in our lab:
- Illumina HiSeq 4000 sequencer (one)
- Illumina HiSeq 2500 sequencers (two)
- Illumina MiSeq sequencers (two)
- Illumina NextSeq 500 sequencer (onetwo)
- Covaris S220 Adaptive Focused Acoustic shearing device
- DigiLab HydroShear shearing device
- Agilent BioAnalyzer 2100
- Agilent TapeStation
- Invitrogen Qubit fluorimeter
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| Terry Heckmann,
Lab Research Assistant
(512) 475-9725 |
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| Brief BioTerry joined the GSAF in August of 2012. Prior to joining the group, Terry had worked for five years as a research technician and lab manager at UT in the College of Natural Sciences and Department of Integrative Biology studying the physiological relationships between migration and reproduction in Melanoplus sanguinipes focusing on the juvenile hormone. Terry has over 37 years of work experience in various clinical, teaching, and academic research labs including Tift General Hospital, The University of Georgia, The University of Oklahoma Health Sciences Center and UT Austin, in fields from human laboratory medicine, parasitology, veterinary surgery and anesthesiology, veterinary radiology, biochemistry and molecular biology, and insect physiology. She has also worked as a biological illustrator and graphic artist. Terry lives with her husband, Peter, and has four children. She enjoys spending time with her family, the symphony, chorale singing, reading, playing classical piano, hiking, gourmet cooking, tennis, and art. |
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| Gabriella Huerta
Research Scientist
(512) 475-9725 |
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