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#!/usr/bin/env bash
#BWA
mkdir -p bwa
bp_seqconvert --from genbank --to fasta <REL606.5.gbk > bwa/REL606.5.fasta
bwa index bwa/REL606.5.fasta
bwa aln -f bwa/SRR030257_1.sai bwa/REL606.5.fasta SRR030257_1.fastq
bwa aln -f bwa/SRR030257_2.sai bwa/REL606.5.fasta SRR030257_2.fastq
bwa sampe -f bwa/REL606.5.sam bwa/REL606.5.fasta bwa/SRR030257_1.sai bwa/SRR030257_2.sai SRR030257_1.fastq SRR030257_2.fastq
#Samtools
mkdir bwa_samtools
cp bwa/REL606.5.sam bwa_samtools/
cp bwa/REL606.5.fasta bwa_samtools/
samtools faidx bwa_samtools/REL606.5.fasta
samtools view -bS -o bwa_samtools/REL606.5.bam bwa/REL606.5.sam
samtools sort bwa_samtools/REL606.5.bam bwa_samtools/sorted_REL606.5
samtools mpileup -uf bwa_samtools/REL606.5.fasta bwa_samtools/sorted_REL606.5.bam \|bcftools view -vcg - \> bwa_samtools/output.vcf
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