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If you are submitting this... | For this platform... | GSAF requests... | Minimum recommended amount |
|---|---|---|---|
Finished NGS sequencing libraries | Illumina HiSeq or MiSeq | 20 ul @ >2 nM | 0.01 picomoles per 1 million reads >50pM |
DNA or RNA | BioAnalyzer QC only | 10 ul > 1 ng/ul | 4 ul > 50 pg/ul |
| DNA - genomic DNA, Must be high molecular weight or the read distributions will not be even | ddRAD genotyping | 25 ul @ 10 ng/ul | 25 ul @ 1 ng/ul |
| DNA - genomic DNA (NOTE: this assumes PURE POPULATIONS - i.e. 100% bacterial or fungal - you may need to assay samples with mixed populations prior to submission) | Metagenomics (bacterial or fungal) | 25 ul @ 10 ng/ul FOR ALL SAMPLES SUBMITTED (i.e. customer must normalize input samples) | 25 ul @ 1 ng/ul |
DNA - genomic DNA, high molecular weight, for library prep | Any platform, fragment library | 5 ug @ > 20 ng/ul | 1 ug @ > 10 ng/ul |
DNA - genomic DNA, high molecular weight, for library prep | Any platform, mate-pair library 1.5-3kb | 100 ug @ > 100 ng/ul | 10 ug @ > 10 ng/ul |
DNA - genomic DNA, high molecular weight, for library prep | Any platform, mate-pair library >3kb | 500 ug @ > 200 ng/ul | 200 ug @ > 100 ng/ul |
DNA fragments (including IP-derived DNA) or DNA amplicons (PCR products), for library prep | Any platform | 500 ng @ > 1 ng/ul | 200 ng @ > 50 pg/ul |
RNA* - total RNA, for library prep | Any platform | 5 ug @ > 50 ng/ul, RIN>7 | 2 ug @ > 5 ng/ul |
RNA* - mRNA, miRNA or other RNA sub-fractions, for library prep | Any platform | 1 ug @ > 10 ng/ul | 100 ng @ > 50 ng/ul |
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