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Please see notes below on guidelines for shipping tubes and plates
If you are submitting this... | Then submit in this...... | For this platform... | GSAF requests... | Minimum recommended amount |
|---|---|---|---|---|
| Finished NGS sequencing libraries | All Final libraries must be in 1.5 ml tubes | Illumina NovaSeq, NextSeq, MiSeq, or iSeq | 20 ul @ >2 nM | |
| DNA or RNA for QC | 1.5 ml tubes | BioAnalyzer QC |
| or Qubit | 10 ul > 1 ng/ul | 4 ul > 50 pg/ul | ||
| DNA - genomic DNA (NOTE: this assumes PURE POPULATIONS - i.e. 100% bacterial or fungal - you may need to assay samples with mixed populations prior to submission) | 96 well plates unless you are submitting 12 or less samples | Metagenomics (bacterial or fungal) | 25 ul @ 10 ng/ul FOR ALL SAMPLES SUBMITTED (i.e. customer must normalize input samples) | 25 ul @ 1 ng/ul |
| DNA - genomic DNA, high molecular weight, for library prep | 1.5 ml tubes, or 96 well plate for 12 or more samples | Any platform, fragment library | 2 ug @ > 20 ng/ul (At least 25 ul's) | 100 ng @ > |
| 5 |
| ng/ul | ||||
| DNA fragments (including IP-derived DNA) or DNA amplicons (PCR products), for library prep | 1.5 ml tubes, or 96 well plate for 12 or more samples | Any platform | 100 ng @ > 1 ng/ul (At least 25 ul's) | 200 ng @ > 50 pg/ul |
| RNA* - total RNA, for library prep | 1.5 ml tubes, or 96 well plate for 12 or more samples | Any platform (Poly-A or Ribosomal Depletion RNA-Seq, TagSeq) | 2 ug @ > 40 ng/ul, RIN>7 (At least |
| 50 ul's) | 500 ng @ > 10 ng/ul | |
| RNA* - mRNA, miRNA or other RNA sub-fractions, for library prep | 1.5 ml tubes, or 96 well plate for 12 or more samples | Any platform |
| 500 ng @ 25 ng/ul (At least 25 ul's) |
| 50 ng @ > 2 ng/ul |
Sample storage solutions
DNA or RNA can be provided in water, TE, low-TE, or other common storage solutions. If you are submitting your sample in something other than water or TE, please note this in either the Sample Description or Brief Job Description fields.
Marking your tubes and preparing to ship
Please write your sample names AND your job number ("JA20xxx") on your tubes before dropping off or shipping to the GSAF lab at MBB 3.210. You will not receive your job number until your electronic submission has been approved by the lab. For any jobs with DNA/RNA for library prep that will have 12 or less samples please submit in 1.5 mL Eppendorf lo-bind tubes, but any 1.5 mL tube is acceptable. Do not send 0.5 ml tubes. For DNA/RNA jobs that need library prep with greater than 12, the samples need to be submitted in a 96 well plate, when submitting your request please upload an excel file with a plate map into the supplemental data section.
If you are shipping your samples do not parafilm them if you have more than 8 tubes. If you have multiple samples we suggest shipping them in some type of box for storage, either plastic or cardboard. 50 ml conical tubes are also acceptable, please do not place your samples loosely among of box full of dry ice for shipment, they should always be in a secondary container.
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Please be sure to write the job number on the side of the plate, if you are submitting multiple plates be sure to label them with a plate number, i.e. plate 1 JA20xxx. You will be required to submit a plate map that references wells to sample names, we ask that you include the actual well position in the sample name, i.e. A1_Test123, B1_Test567 ect...The plate map can be uploaded using the supplemental upload function when submitting the job.
Please use skirted or semi-skirted plates, and seal with a foil. Place a piece of cardboard or some protective barrier over the top of the foil for protection if shipping the samples. We would strongly recommend placing each plate inside its own box even if you are shipping multiple plates, be sure to thoroughly seal the plates!!
Samples should be plated starting at A1 then plate the samples vertically, A1 to H1, then A2 to H2 and so on.....Do not skip wells, you will be charged for wells that are skipped and we will not process samples that are plated A1 to A12.
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