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Please see notes below on guidelines for shipping tubes and plates
If you are submitting this... | Then submit in this... |
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... | For this platform... | GSAF requests this amount... |
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Minimum recommended amountIn this volume... |
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| Finished NGS sequencing libraries | All Final libraries must be in 1.5 ml tubes | Illumina NovaSeq, NextSeq, MiSeq, or iSeq |
20 ul @ >2 nM| >2nM | ≥20ul |
| DNA or RNA for QC | 1.5 ml tubes |
BioAnalyzer QC or Qubit | 10 ul > 1 ng/ul | 4 ul > 50 pg/ul | Bioanalyzer-DNA | >50pg/ul | 3-5ul |
| Bioanalyzer-RNA Nano | 25-500ng/ul (Total RNA) 25-250ng/ul (mRNA) |
| Bioanalyzer-RNA Pico | .05-5ng/ul (Total RNA) .25-5ng/ul (mRNA) |
| Bioanalyzer-small RNA | 1-100ng/ul (Total RNA) 1-20ng/ul (enriched small RNA) |
| DNA - genomic DNA (NOTE: this assumes PURE POPULATIONS - i.e. 100% bacterial or fungal - you may need to assay samples with mixed populations prior to submission) | 96-well plates |
unless | *, or 1.5mL tubes if you are submitting |
12 or less | ≤12 samples | Metagenomics (bacterial or fungal) |
25 ul @ 10 ng| 10ng/ul FOR ALL SAMPLES SUBMITTED (i.e. customer |
must | MUST normalize input samples) |
25 ul @ 1 ng/ul | ≥25ul |
| DNA - genomic DNA, high molecular weight, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* |
Any platform, fragment library | 2 ug @ > 20 ng/ul (At least 25 ul's) | | DNA library prep, Covaris fragmentation | 1-80ng/ul | ≥25ul |
100 ng @ > 5 ng/ul |
| DNA fragments (including IP-derived DNA) or DNA amplicons (PCR products), for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* |
Any platform | 100 ng @ > 1 ng/ul (At least 25 ul's) | 200 ng @ > 50 pg/ul | | DNA library prep | 1-80ng/ul | ≥25ul |
| RNA |
RNA* | - total RNA, for library prep** | 1.5 ml tubes, or 96-well plate for 12 or more samples |
Any platform (Poly-A or Ribosomal Depletion | * | RNA-seq, PolyA purification | 10-80ng/ul | ≥25ul |
| RNA-seq, Ribosomal Depletion | 2-80ng/ul | ≥25ul |
| RNA-Seq, |
TagSeq)2 ug @ > 40 ng/ul, RIN>7 (At least 50 ul's) | 500 ng @ > 10 ng/ul | | no removal or enrichment | 1-200ng/ul | ≥15ul |
| TagSeq | 10-100ng/ul (samples MUST be normalized within this range) | ≥25ul |
| RNA-mRNA, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* | RNA-Seq | .2-20ng/ul | ≥15ul |
| RNA- |
RNA* - mRNA, | miRNA or other RNA sub-fractions, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples |
Any platform | 500 ng @ 25 ng/ul (At least 25 ul's) | | * | Small RNA library prep | 20-200ng/ul(Total RNA) 2-20ng/ul (purified small RNA) | ≥15ul |
* Samples should be plated starting at A1 then plate the samples vertically, A1 to H1, then A2 to H2 and so on.....Do not skip wells, you will be charged for wells that are skipped and we will not process samples that are plated A1 to A12. ** RNA must be free of DNA |
50 ng @ > 2 ng/ul Sample storage solutions
DNA or RNA can be provided in water, TE, low-TE, or other common storage solutions. If you are submitting your sample in something other than water or TE, please note this in either the Sample Description or Brief Job Description fields.
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