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Let's recap what we learned yesterday:

We looked at finding differentially expressed genes when we are not interested in novel genesmapping reads to the genome and transcriptome.

 

       1. Unspliced mapping: BWA. We used BWA to map reads from two conditions C1 and C2 to our genome.

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    • When you want to quantify known transcripts, kallisto is the fastest way to go. It skips the alignment step.

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