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Now that we have a bam file with only the reads we want included, we can do some more sophisticated analysis using bedtools.  Bedtools changes from version to version, and here we are using version 2.22, the newest version, and what is currently installed on stampede.

 

Exercise 1: convert bam to fastq and look at the quality scores

Exercise 2: Convert bam to bed using bamtobed

Exercise 3: Find the coverage of your bed file over the genome

Exercise 4: Intersect two experiment using intersect

Exercise 5: Use bedtools merge to merge two experiments

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