About Us

The GSAF is a full service sequencing core that provides both Sanger and Next Generation Sequencing services, in addition to a library prep and quality check services, open access to equipment and training.  The GSAF works directly with the bioinformatics core who can provide assist in any of your analysis needs to data storage solutions.

We are housed in approximately 2,000 square feet of controlled-access laboratory space and are an experienced NGS facility. Launched in 2008, the GSAF currently processes over 10,000 NGS samples per year.

Instruments in our lab:

• Illumina NovaSeq 6000 (one)
• Illumina MiSeq sequencers (three)
• Illumina NextSeq 500 sequencer (two)
• Covaris S220 and E220 Adaptive Focused Acoustic shearing devices
• Agilent BioAnalyzer 2100
• Agilent TapeStation
• Invitrogen Qubit fluorimeter and Thermoscientific Fluoroskan
• Tecan Evo and Hamilton Nimbus
• 10x Chromium system
• Ion Torrent S5

Lab protocols we are experienced with:

• Creation of fragment DNA sequencing libraries for all Illumina next-gen sequencers
• Creation of mate-pair sequencing libraries between 1.5kb and 4kb for Illumina next-gen sequencers
• Creation of RNA-seq libraries from total RNA, small RNA, and immuno-precipitated RNA for Illumina next-gen sequencers
• Creation of amplicon libraries (particularly 16s and ITS regions) for the Illumina next-gen sequencers
• Human exome and custom capture with the Agilent SureSelect, Illumina TruSeq, and Nimblegen SeqCap EZ kits
• Sample and library QC using the Agilent BioAnalyzer, Picogreen and Ribogreen fluorimetry, qPCR, and spectrophotometry

Computational and software resources:

• The bioinformatcis group maintains a wide range of tools for NGS analysis and assembly and can assist with all of your analysis needs, please visit them here. 
• Need your own server or access to one of ours, here is an explanation of the Biomedical Research Computing services with instructions for getting an account.
• In addition, the GSAF uses and works with the TACC bioinformatics group, supporting tools and applications suitable to the TACC environment.
• Want to get started? Contact us
• New to Unix? Check out some Unix and Perl resources for beginners.
  
  

Our Mission and Vision

Vision: The GSAF’s vision is to be a world-class genomic analysis center in terms of data quality, breadth of available methods, and productivity.

Mission: The mission of the Genome Sequencing and Analysis Facility is to provide the best quality analytical results with the best value to life science researchers.

Our People