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Create output directory.

mkdir -p 03_Output/variant_calling/samtools

Preparing alignment file.

If you do not have a alignment file in the SAM format you may want to start with Introduction to mapping.

samtools view -bS -o 03_Output/variant_calling/samtools/REL606.5.bam 03_Output/mapping/bowtie/REL606.5.sam
[bam_header_read] EOF marker is absent. The input is probably truncated.
[bam_header_read] invalid BAM binary header (this is not a BAM file).
[main_samview] fail to read the header from "03_Output/mapping/bowtie/REL606.5.sam".
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