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A video post on methods! Today Chloe Wooldridge and Juan P. Maestre talking about the SKC Biosampler.
According to wikipedia a bioaerosol "is a suspension of airborne particles that contain living organisms or were released from living organisms". These particles vary in size, from nanometers (viruses), micrometers (bacteria and fungi), to hundred micrometers (pollen particles). Bioaerosols can be collected by means a variety of devices such as collection plates, electrostatic collectors, and impactors.
The SKC Biosampler is an impactor, part of the so-called impinger samplers. These kind of samplers impact bioaerosols into a liquid, such as phosphate buffer solution (PBS), that swirls upward on the sampler's inner wall and collects the particles. According to SKC, the BioSampler is a highly efficient collector and only requires a high-volume sonic flow pump to trap airborne microorganisms.
Here the video on how to use this device.
I hope you like it!
Here for your reference, a couple of papers using this device: 1 and 2.
Dear students, here you have the information about the Relative Humidity and Temperature for indoor and outdoor.
Here the link to download the data:
Here two schemes representing the locations where some of the samples were withdrawn.
Let me know if you have any question. Follow updates on Twitter @UTBiome, and LIKE US on Facebook https://www.facebook.com/UTBiome .
Thanks Harish Sangireddy for your help with the schemes!
I just want to give you an update regarding the samples we took on Tuesday and the metadata associated. The metadata is being compiled and curated to make sure all data were correctly annotated. The samples have been processed and are kept frozen at -20C. In the next 24 hours some of the samples will be DNA-extracted. Afterwards we will be doing qPCR to estimate total bacterial load in the selected samples. If any of you want to come over the lab and collaborate, please let us know. It will be a pleasure to show you the lab and the procedures.
Follow updates in twitter @UTBiome, in facebook UTBiome and here.
JP.
Today the students from CE369L have been sampling in class (oh well, we started by 7am). The 11 groups of students were sampling not only in the actual classroom but also in the corridor, the adjacent hall and outside. Bioaerosol samples were taken indoor and outdoor by using SKC impingers and Button Samplers. A baseline was established before the students came in and the "in class" measurements started as early as 8am. Sleepy faces could be observed around .
Dust had been collected in plates for 5 days. A variety of surfaces were wiped and swabbed too. The biological samples were complemented with #metadata: CO2, particle counts, surface temperatures, relative humidity and temperature, air flowrates (for the whole class and on the sample locations) and pressure differences (between class and corridor). Here some pictures of the sampling event.
It was very fun and instructive. A great experience!