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Post-translational modification (PTM) identification

Identify and analyze PTMs in large-scale, 12 hour minimum ($564 internal, $720 external) per project.

1. List of modified and unmodified peptides with mass-shifts

Identify modified peptides along with the unmodified counterparts.

Deliverables:
- reports generated by MODa (mass-shifts and peptide-spectral matches)

Tools Used:
- MODa: (http://prix.hanyang.ac.kr/download/moda.jsp) used to identify mass-shifts on given peptides

2. Mapping mass-shifts to post-translational modifications and substitutions

Mapping the mass-shifts identified in step 1. to actual post-translational modifications and/or substitutions

Deliverables:
- Mass-shift to PTM mapping

Tools Used:
- ABRF delta mass: (https://www.abrf.org/index.cfm/dm.home?AvgMass=all ) used to identify PTMs mapping to mass-shifts identified above (i.e., 80 Dalton mass-shift on Serine indicates phosphorylation)

3. Comprehensive list of all modified peptides

List of all peptides mapped to proteins along with the site of modification (i.e., Serine phosphorylation)

Deliverables:
- Excel file that has list of all modified peptide-spectral matches