Prices shown below are estimates based on typical pricing. According to UT System and UT Austin policy, the GSAF bills for actual expenses can vary with the size and scope of each project. The GSAF does not guarantee read counts but we will try our best to meet customer request when we prepare the libraries, however we will not make up any read counts for customer prepared libraries.
10X Library Preparation | Cost per sample Internal | Cost per sample External |
1 Sample, Library Prep Only, Gene Expression v3.1 or Immune Profiling v2 | $2303.53 | $2917.18 |
Each additional sample, up to 4 total, Library Prep Only | $2047.01 | $2579.24 |
Add Feature Barcode (must use compatible 10X partners) | $255.15 | $321.49 |
Add TCR or BCR Amplification | $238.90 | $301.01 |
Add both FB and TCR/BCR Amplification | $494.05 | $622.50 |
Tag-Seq Cost (Library Prep and Sequencing, NovaSeq 6000 SR100) | Cost per sample Internal | Cost per sample External |
Standard Coverage (3-5 M reads) | $60 | $75 |
High Coverage (7-10 M reads) | $83 | $105 |
Tag-Seq Cost (Library Prep and Sequencing, NextSeq 75) | Cost per sample Internal | Cost per sample External |
Standard Coverage (3-5 M reads) | $79 | $99 |
High Coverage (7-10 M reads) | $121 | $153 |
**IF min sample number is not met $240 added for internal and $303 for external (20 Sample Minimum)
DNA ~400 bp insert size. Price includes final QC by BioAnalyzer and qPCR. See this web page for sample input guidelines.
RNA Size range typically ~200 nt; "dUTP" directional protocol. Price includes QC by BioAnalyzer on final lib only and qPCR. Please request a BA if you need to check the quality of the RNA before we proceed to library prep. See this web page for sample input guidelines.
Prices listed are per sample. For projects with 8 or less samples, a flat fee per project is charged as shown.
Type of Library Preparation Procedure | Internal / UT Network | External Academic |
Standard DNA library | $101 | $127 |
RNA directional WITH poly-A enrichment | $206 | $260 |
RNA directional WITH ribosomal removal | $301 | $381 |
Small RNA or RNA with no poly-A or ribosomal removal | $141 | $179 |
Contact the GSAF and we will be happy to plan out a project for your research goals.
The cost for outer PCR is about $30.50 a sample. You can follow this design to produce amplicons compatible with our indexing oligos.
Please note, due to increased sequencing cost and library prep reagents the cost of this prep has increased.
Metagenomics assay: This service starts with normalized (i.e. equal concentration and volume) gDNA and includes amplification using one of the GSAF provided primer sets chosen by the customer as shown on this web page. The service is calibrated to deliver at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 90% of the samples submitted, assuming there is reasonable amount of DNA to amplify. To minimize jackpot effects, PCR is performed in triplicate for each sample and re-pooled.
The library preparation is a two-step process, a gene-specific PCR then a second PCR to add the dual indexes. The PCR targets the bacterial 16S V4/V5 region, the 16S V4 region, or fungal ITS region, as specified by the client. Gel-based QC is performed on a sampling of 10% of the samples post amplification. Genomic DNA is expected as input; the GSAF does not provide DNA extraction services at this time. See this web page for sample input guidelines and note that DNA concentrations should be normalized before submission. The GSAF can normalize your sample concentrations for an additional charge.
Prices are listed per sample to include library preparation from genomic DNA and sequencing. For jobs with less than 96 samples, a flat fee per project is charged as shown.
Prices vary slightly depending on the actual number of samples processed, so the prices are provided for reference and planning purposes. Submit a job request to get accurate, approved pricing.
Number of Samples | Internal / UT Network | External Academic |
96 or more | $42.70 | $53.90 |
1-95 samples | Add $369 / project | Add $464 / project |
IMPORTANT CAUTION: The V4/V5 region assay we use is KNOWN to amplify Eukaryotic 18S rRNA genes as well as bacterial 16S V4/V5 so choose your primer sets carefully.
Note: if you are submitting a mixed population (e.g. bacterial & fungal, or bacterial & eukaryote host, etc.) you should assay your samples before submission to ensure you have the proper concentration of the target DNA, not simply total DNA. Samples submitted with insufficient target DNA may result in excessive primer-dimer or off-target products which produce no useful data. These will still be charged according to the pricing table.