Day 1: Introduction to RNA-Seq and Read Mapping

• Part 1: Introduction to RNA-Seq
• Part 2: TACC Refresher

• TACC Overview
• Using The Lonestar Cluster
• Lonestar directory structure
• Submitting jobs to Lonestar

• Part 3: Read Mapping
  • Introduction to mapping
  • Mapping with BWA
  • Mapping with Tophat2
  • Assessing mapping results