Protein Identification and Validation
Identify and validate peptides/proteins given raw mass-spec data
1. Protein identification using Trans-Proteome Pipeline
Identify list of proteins in the sample using multiple search algorithms (a) Identify peptides from raw mass-spec data (using multiple search engines that are available in Trans-Proteome Pipeline (TPP)
(b) Peptide level validation (using PeptideProphet)
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- Deliverables:
- List of all peptide-spectral matches ( no Post Translational Modifications identification). For PTM Identification, see Post-translational modification (PTM) identification
- Tools Used:
- Trans-Proteome Pipeline: (Deutsch E. W. et. al. )
- XML output of all peptide and protein hits.
- Trans-Proteome Pipeline: (Deutsch E. W. et. al. )