Fragment Analysis
The AB 3730 and 3730XL are also used for the analysis of microsatellites, AFLP, tRFLP, SNPs, and other fragment applications.
Fluorescently labeled fragments are detected using the Applied Biosystems 3730 Genetic Analyzer and interpreted using GeneMapper or GeneMarker analysis software. Up to five different colored fluorescent dyes can be detected in one sample. One of these fluorescent dyes is used as a labeled size standard and added to each lane to allow comparison of samples from lane to lane, or gel to gel. The analysis software determines the length of each dye-labeled fragment based on the standard curve generated for each specific lane. Accuracy of less than one base difference can be observed between sample replicates. Higher throughput can be achieved by multiplexing different dyes and fragment sizes together.
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