Overviews of Harris Lab Methods:
- Harris KM, Perry E, Bourne J, Feinberg M, Ostroff L, and Hurlburt J (2006) Uniform serial sectioning for transmission electron microscopy. J Neurosci 26(47):12101-12103. (PDF) (Supplemental movies)
- Kuwajima M, Mendenhall JM, Harris KM (2013) Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy. Methods Mol Biol (Nanoimaging: Methods and Protocols) 950:253-73. (PDF)
Experimental Protocols:
- Acute Hippocampal Slices
- Synchroslice
- Microwave-assisted Chemical Fixation of Acute Hippocampal Slices
- WESCOR VAPRO 5520 Osmometer
- Perfusion-fixation of rats and mice
- Agarose Embedding and Vibraslicing Fixed Brain Tissue
- Pre-embedding Immunohistochemistry
- Epoxy Embedding of Fixed Brain Tissue
- Imaging Blocks with the Leica Stereoscope
- Coating TEM Grids with Polyetherimide or Pioloform F
- Ultramicrotome and Serial Sectioning
- Post-embedding Immunogold Labeling
- Post-section Staining of Ultrathin Sections with Uranyl Acetate and Lead Citrate
- tSEM Imaging
- TEM Imaging
- EM Tomography
- Also see here.