Title: Experimental Protocols  
Author: Masaaki Kuwajima Mar 14, 2016
Last Changed by: Lyndsey Michelle Kirk Apr 15, 2024
Tiny Link: (useful for email) https://wikis.utexas.edu/x/j5MEC
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KH Lab Wiki (1)
    Home page: KH Lab Wiki Home
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    Home page: KH Lab Wiki Home
Children (25)
    Page: Transcardiac Perfusion
    Page: WESCOR VAPRO 5520 Osmometer
    Page: Microwave-assisted Chemical Fixation of Acute Hippocampal Slices
    Page: Epoxy Embedding of Fixed Brain Tissue
    Page: Imaging Specimen Blocks with the Leica M125 Stereoscope
    Page: Coating TEM Grids with Polyetherimide
    Page: Coating TEM Grids with Pioloform F
    Page: Ultramicrotome and Serial Sectioning
    Page: Staining Ultrathin Sections with Uranyl Acetate and Lead Citrate
    Page: tSEM Imaging
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  • Only users in group khlab-senior can edit this page. (set by Masaaki Kuwajima at Aug 11, 2016 10:15)
  • Only users in group khlab-super can edit this page. (set by Masaaki Kuwajima at Nov 28, 2017 04:22)
  • Only users in group khlab-user can edit this page. (set by Masaaki Kuwajima at Nov 27, 2017 09:07)
  • Only mk23564 can edit this page. (set by Masaaki Kuwajima at Nov 27, 2017 09:07)
Recent Changes
Time Editor  
Apr 15, 2024 12:24 Lyndsey Michelle Kirk View Changes
Apr 15, 2024 12:23 Lyndsey Michelle Kirk View Changes
Aug 02, 2023 15:44 Masaaki Kuwajima View Changes
Aug 02, 2023 15:39 Masaaki Kuwajima View Changes
May 05, 2023 14:06 Katy Pilarzyk  
Outgoing Links
External Links (11)
    https://www.fishersci.com/shop/products/water-acs-reagent-g…
    https://wikis.utexas.edu/x/4BSVFg
    synapseweb.clm.utexas.edu/protocols/
    www.jneurosci.org/content/26/47/12101/suppl/DC1
    synapseweb.clm.utexas.edu/alignment-tem
    https://wikis.utexas.edu/display/khlab/Cryoprotecting+Tissu…
    https://doi.org/10.1523/JNEUROSCI.3994-06.2006
    https://doi.org/10.1007/978-1-62703-137-0_15
    synapses.clm.utexas.edu/sites/default/files/synapseweb/file…
    synapseweb.clm.utexas.edu/loadingunloading-gimbols-rotation…
    synapses.clm.utexas.edu/sites/default/files/synapseweb/file…
KH Lab Wiki (29)     Page: A compilation of previously used tissue processing protocols
    Page: Ultramicrotome and Serial Sectioning
    Page: en bloc staining and epoxy embedding of fixed brain tissue
    Page: High-pressure Freezing and Freeze-Substitution
    Page: Shipping fixed brain tissue for EM processing to the Harris lab
    Page: Using Zeiss Axio Imager.Z2 (NHB 4.228)
    Page: Transcardiac Perfusion
    Page: en bloc only staining and epoxy embedding of chemically fixed tissue (70-250 µm thick tissue)
    Page: tSEM Imaging
    Page: Coating TEM Grids with Pioloform F
    Page: WESCOR VAPRO 5520 Osmometer
    Page: Toluidine Blue staining for semi-thin sections
    Page: Notes on slicing-induced synaptogenesis
    Page: Using Zeiss microscopes (NHB 3.xxx)
    Page: Staining Ultrathin Sections with Uranyl Acetate and Lead Citrate
    Page: mAPEX2-related protocols
    Page: Experimental Protocols
    Page: Epoxy Embedding of Fixed Brain Tissue
    Page: Using Zeiss Axio Observer 5 microscope
    Page: Immunofluorescent Labeling
    Page: Osmiun-Free Processing and Epoxy Embedding of Fixed Brain Tissue
    Page: Microwave-assisted Chemical Fixation of Acute Hippocampal Slices
    Page: Long-term storage of chemically fixed vibratome-sectioned brain tissue
    Page: Imaging Specimen Blocks with the Leica M125 Stereoscope
    Page: Coating microscope slides with gelatin
    Page: Agarose Embedding and Vibraslicing Fixed Brain Tissue
    Page: Rapid decapitation and brain cryopreservation
    Page: Cresyl Violet staining
    Page: Coating TEM Grids with Polyetherimide