Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

The GSAF is a full service sequencing core that provides both Sanger and Next Generation Sequencing services, in addition to a library prep and quality check services, open access to equipment and training.  The GSAF works directly with the bioinformatics core who can provide assist in any of your analysis needs to data storage solutions.


We are housed in

...

bordertrue

Our Resources

The Genomic Sequencing and Analysis Facility at the University of Texas at Austin is located in room 4.102 of the MBB building, which houses the ICMB. We perform next-gen sequencing and microarray studies for UT researchers from any department and from external research entities, both public and private. We are supported by UT's Texas Institute for Drug & Diagnostic Development and the Center for Systems and Synthetic Biology.

In approximately 2,000 square feet of controlled-access laboratory space and are an experienced NGS facility. Launched in 2008, our facility currently includes:

...

the GSAF currently processes over 10,000 NGS samples per year.

Instruments in our lab:

  • Illumina NovaSeq 6000 (one)
  • Illumina MiSeq sequencers (three)
  • Illumina NextSeq 500 sequencer (two)
  • Covaris S220 and E220 Adaptive Focused Acoustic shearing devices
  • Agilent BioAnalyzer 2100
  • Invitrogen Qubit fluorimeter and Thermoscientific Fluoroskan
  • Tecan Evo and Hamilton Nimbus
  • 10x Chromium system

Lab protocols we are experienced with:

  • Creation of fragment DNA sequencing libraries for all Illumina next-gen sequencers
  • Creation of RNA-seq libraries from total RNA, small RNA, and immuno-precipitated RNA for Illumina next-gen sequencers
  • Creation of amplicon libraries (particularly 16s and ITS regions) for the Illumina next-gen sequencers
  • Human exome and custom capture with the Agilent SureSelect, Illumina TruSeq, and Nimblegen SeqCap EZ kits
  • Sample and library QC using the Agilent BioAnalyzer, Picogreen and Ribogreen fluorimetry, qPCR, and spectrophotometry

Computational and software resources:

Anchor
vision
vision

Our Mission and Vision

Mission: The Genomic Sequencing and Analysis Facility mission is to provide the community with a level of technical expertise and experience that will support ongoing research projects. We aim to provide the highest quality data, develop strong customer relationships through individualized support, and provide educational opportunities that focus on the rapidly evolving field of genomics.

Vision: Support state of the art equipment and expand our services supporting research by incorporating new methodologies and techniques into the core to advance research.

Values: Our core facility is dedicated to the researchers we serve and the staff members that make the facility a success.  We promote strong collaborations with researchers always welcoming the experienced and novice scientist, focus on education opportunities for the customers we support and continuing education for our staff and operate at the highest level of integrity with full transparency. 

...

Our People

Section
bordertrue


Column
width12%

Image RemovedImage Added


Column
width21%

Jessica Wheeler Podnar
Director: Scott Hunicke-Smith, Ph.D.
(512) 232-5924
MBB 23.406210
scotth@mailgsaf@utgsaf.utexas.eduorg


Column
width67%

Dr. Hunicke-Smith joined UT Austin in August of 2008 to start next-generation sequencing activities at UT. Prior to joining UT, he was VP and General Manager of the molecular biology Services’ division of Asuragen, a spin-out of Ambion. He joined in Ambion in July of 2003 as Director of Business Development, Instrumentation. He was promoted to Vice President, Business Development in March of 2004 and to Vice President, Ambion Services in September of 2005. Prior to joining Ambion, Dr. Hunicke-Smith served as founder and CEO of GeneMachines, a molecular biology equipment firm, from 1997 through March of 2003. Dr. Hunicke-Smith also served on the Board of Directors of Silicon Genetics, a bioinformatics software firm, from 1998 until Agilent acquired it in 2004. He received his Ph.D. from Stanford University in the laboratory of Ron Davis in 1997 and is an inventor on seven issued patents.Jessica is a graduate of Southwest Texas State University and began her career in cell and molecular biology over 17 years ago starting at a biotech company in Reno, NV focused on telomere biology.  Her next endeavor led to the Genomic Sequencing Facility where she began as the Lab Manager in June 2010, as her role continued to grow and expand so did her responsibilities which eventually lead to the opportunity to serve as the Director. Her years of experience covers a wide range of applications, from protocol development and optimization, the many transitions in NGS platforms and preps, lab and personnel management, establishing pricing structures and policies for the facility, all the way to setting up COVID testing laboratory for the university.  She is also an active member of the Association for Biomolecular Resource Facilities and serves as the chair for the DNA Sequencing Research Group.  




Section
bordertrue


Column
width12%

Image RemovedImage Added


Column
width21%

Gabriella Huerta
Program ManagerTitle: Bioinformatician
(512) 471475-7138
MBB 2.404
darasappan@mail.utexas.edu9725


Column
width67%

Dhivya joined the GSAF team in April 2008 and primarily deals with analysis of Next-gen sequencing data. Prior to joining UT, she was working at the National Center of Toxicological Research, FDA. At FDA, she was involved in analysis of gene expression data. She has a Masters in Bioinformatics from Virginia Commonwealth University and a Bachelors in computer science from Anna University, IndiaGabriella manages the Illumina sequencing operations and contributes to all aspects of the genomic sequencing enterprise.  Every library barcode, concentration and quality control metric is carefully scrutinized by Gabriella, as she performs the complex task of setting up the sequencing runs to assure the highest quality results.  With prior experience running NGS operations at Perlegen Sciences and Pfizer, Gabriella has the depth of experience to get the jobs done right.



Section
bordertrue


Column
width12%

Image RemovedImage Added


Column
width21%

Title: Laboratory Manager
(512) 475-9725
MBB 4.102
jessicawheeler24@gmail.com

Holly Stevenson
Research Engineering Scientist Associate IV


Column
width67%

Brief BioHolly is a PhD scientist with prior experience applying and tailoring molecular protocols to cancer genetics research during her time as a graduate student and postdoc.  Her interests expanded to include genomics and she subsequently served as a Biologist in a core facility for 5 years at the National Cancer Institute, preparing NGS libraries and implementing microarrays for both research and clinical studies.  Since joining GSAF in 2017, she has assisted in testing and expanding new services, including TagSeq and 10X Genomics single-cell technologies.  She currently operates the Illumina Novaseq sequencer, manages and performs runs for single-cell studies, and aids customers in project planning and troubleshooting.



Section
bordertrue


Column
width12%

Your photo goes hereImage Added



Column
width21%

Heather Deiderick
Title: Post-doctoral researcher
(512) 475-9725

Andrew Villarreal
Administrative Associate


Column
width67%

Brief BioAndrew is a University of Texas at Austin alumnus who joined the CBRS team in 2015. He has over 15 years of experience in business operations, shipping, and receiving. He provides support by processing invoices, internal vouchers, and purchasing for Computational Biology and Bioinformatics, Biomedical Imaging Center, DNA Sequencing Facility, and Genomic Sequencing and Analysis Facility




Section
bordertrue


Column
width12%

Your photo goes hereImage Added



Column
width21%

Heather Jefts
Title: Lab Research Assistant
(512) 475-9725

Aditi Karmakar
Research Engineering Scientist Associate III


Column
width67%

Brief BioAditi has a master's degree in Genetic Engineering from India and has over 11 years of experience in molecular biology techniques. She has been under UT community for 5 years, and previously she has extensive experience in understanding and handling DNA and RNA for various Research purposes both in and outside UT. She was looking at how different environmental factors like; diet and alcohol can affect CNS inflammatory disorders like Multiple sclerosis in Melamed lab in UT and that experience gave her an immense knowledge on various molecular mechanisms as well as the variety of techniques that are used to study them. She has also worked as one of the technicians in Sanger core and processed samples for sequencing and Fragment analysis.   She is currently involved in a variety of DNA/RNA library preparations and operating our Illumina Miseq sequencers.  




Section
bordertrue


Column
width12%

Your photo goes hereImage Added


Column
width21%

María Jose Gomez
Research Engineering Scientist Associate IIJillian DeBlanc
Title: Lab Research Assistant
(512) 475-9725


Column
width67%

Brief BioMaría attended the Universidad de los Andes in Colombia where she completed her studies on Biology with a minor in Bioinformatics.  Before joining the GSAF her work included a variety of molecular techniques on systems such as rodents, frogs, bacteria, viruses and plants.  As part of the GSAF her focus is on NGS library preparation, including DNA libraries, RNA-Seq, TagSeq and quality control of said libraries.





Section
bordertrue


Column
width12%

Your photo goes hereImage Added


Column
width21%

Melanie Weiler
Title: Undergraduate Research Assistant, Laboratory
(512) 475-9725John Ludes-Meyers
Research Engineering Scientist Associate IV


Column
width67%

Brief Bio

Section
bordertrue
Column
width12%

Your photo goes here

Column
width21%

Joe Cruz
Title: Undergraduate Research Assistant, Bioinformatics
(512) 475-9725

Column
width67%

Brief Bio

John joined the GSAF after over 25 years of academic research focusing on molecular mechanisms of carcinogenesis.  John’s passion for molecular genetics began while studying RNA tumor viruses as an undergraduate at the University of Minnesota.  He continued studying viruses as a graduate student at Texas A&M University working on papillomavirus replication.  His post-doctoral work started a long stint of cancer biology research leading to a decade of work as a Research Scientist at MD Anderson Cancer Center-Science Park studying the novel tumor suppressor, WWOX.  After a short break from science to experience the world of software development at National Instruments, John came back to scientific research at UT.  John has a wide spectrum of technical experience in molecular and cellular biology.  As a member of GSAF John will apply his expertise to NGS library construction, single-cell gene expression and DNA methylation array services.








Section
bordertrue


Column
width12%

Your photo goes hereImage Added


Column
width21%

Title: Name
Phone numberZachary Carver
Research Engineering Scientist Associate II


Column
width67%

Brief Bio

Image Removed

Alumni

Section
bordertrue
Column
width12%

!!

Column
width21%

Name:

Column
width67%

BioNEED BIO