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- TagSeq is a new service offered by the GSAF, this is a 3' RNA based library prep utilizing the intrinsic properties of a reverse transcriptase
- RNA submitted must be high quality and should be normalized, preffered concentration for the RNA is between 10-100 ng/ul providing at least 25 microliters
- Samples must be submitted in 96 well plates
- If Trizol/Tri-Reagent was used for extraction samples should go through a column clean up
Tag-Seq Cost (Library Prep and Sequencing, HiSeq 2500 SR50) | Cost per sample Internal | Cost per sample External |
Standard Coverage (3-5 M reads) | $61.46 | $77.75 |
High Coverage (7-10 M reads) | $87.76 | $111.02 |
Tag-Seq Cost (Library Prep and Sequencing, HiSeq 2500 SR100) | Cost per sample Internal | Cost per sample External |
Standard Coverage (4-5 M reads) | $70.81 | $89.57 |
High Coverage (8-10 M reads) | $106.46 | $134.67 |
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