Sanger Sequencing
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 10 uL of sample (12-15 uL preferred)
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 10 uL of sample (12-15 uL preferred)
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the plate and foil
- Put your samples in order vertically, starting with well A1
What NOT to do!
Do not pool like samples. 1 tube = 1 sequencing reaction!
Do not put your sample NAME on your tubes
Do not give us your primer (unless you are requesting PCR cleanup)
Do not use 0.5 mL microfuge tubes
Do not submit orders of >48 samples in individual tubes Do not ask us if we will run your samples after 10:00 A.M.
Thank you for your cooperation!PCR Cleanup
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 20 uL of unpurified PCR reaction
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap.
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 20 uL of unpurified PCR reaction
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the plate and foil
- Put your samples in order vertically, starting with well A1
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap
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