The DNA Sequencing Facility provides Sanger DNA sequencing, fragment analysis, quantitative real-time PCR, automated liquid handling and instruments that researchers can use for quantification and imaging.
Automated DNA sequencing is performed using capillary-based Applied Biosystems 3730 DNA Analyzers. These instruments offer high throughput and sensitivity with a capability of handling more than 1400 samples per day, with reads greater than 700 base pairs and a success rate of over 90 percent. The AB 3730 and 3730XL are also used for the analysis of micro-satellites, AFLP, SNPs,tRFLPs and other fragment applications.
Quantitative real-time PCR is run on an Applied Biosystems ViiA 7s or QuantStudio. These instruments allow researchers to analyze gene expression using allelic discrimination and SNP analysis in 96 or 384 wells.
Other instruments are available for quantification, phosphor and fluorescent imaging, a plate reader, and automated liquid handling. The NanoDrop spectrophotometer is used for quantification of DNA, RNA, and proteins using only one or two microliters without a cuvette. The Typhoon Trio Imager measure and image radioactive signals using from gels or membranes using phosphor screens and fluorescence from gels, membranes, TLC plates, and microtiter plates. The QIAgility, Biomek NX and Biomek FX robots, and are used for automated pipetting applications. The NightOwl is available for low-light imaging of luminescence or fluorescence in plants or animals.
