The DNA Sequencing Facility is proud to offer the following services to The University of Texas at Austin research community, as well as many UT academic affiliates, liberal arts colleges, biotech companies, and government institutions in Texas and beyond. Please contact us at dsfcore@austin.utexas.edu or 512-475-7844 if you are interested in using our services.
Sanger sequencing
Fragment analysis
Quantitative real-time PCR
Gradient PCR
Automated liquid handling
Instruments for quantification and imaging of DNA, RNA, and proteins
Custom services, such as CRISPR and SNP analysis
Please note: If you are interested in Next Generation Sequencing (NGS) services, please contact our Genomic Sequencing and Analysis Facility at gsaf@utgsaf.org or 512-475-9725.
Automated DNA sequencing and fragment analysis are performed using capillary-based Applied Biosystems 3730 DNA Analyzers. These instruments offer high-throughput and sensitivity, allowing same day results for 800 samples per day. We can sequence ssDNA, dsDNA, and PCR products, as well as analyze microsatellite, AFLP, tRFLP, and SNP fragments. Quantitative real-time PCR is run on Applied Biosystems ViiA 7 instruments using QuantStudio software. These instruments allow researchers to analyze gene expression using allelic discrimination and SNP analysis in 96 or 384 wells. Several other instruments are available for quantification, phosphor and fluorescent imaging, and automated liquid handling. The NanoDrop spectrophotometer is used for quantification of DNA, RNA, and proteins using only one or two microliters without a cuvette. The Typhoon Trio Imager measure and image radioactive signals using from gels or membranes using phosphor screens and fluorescence from gels, membranes, TLC plates, and microtiter plates. The QIAgility, Biomek NX and Biomek FX robots, and are used for automated pipetting applications. The NightOwl is available for low-light imaging of luminescence or fluorescence in plants or animals.
