The DNA Sequencing Facility provides DNA sequencing, fragment analysis, quantitative real-time PCR, NanoDrop spectrophotometer, phosphor and fluor imaging, a plate reader, and automated liquid handling. Automated DNA sequencing is performed using capillary-based Applied Biosystems 3730 DNA Analyzers. These instruments offer high throughput and sensitivity with a capability of handling more than 1400 samples per day, with reads greater than 700 base pairs and a success rate of over 90 percent. The AB 3730 and 3730XL are also used for the analysis of microsattelites, AFLP, SNPs,tRFLPs and other fragment applications. Quantitative real-time PCR is run on an Applied Biosystems ViiA 7s. These instrument allow researchers to analyze gene expression using allelic discrimination and SNP analysis in 96 or 384 wells. The quantification of DNA, RNA, and proteins using only one or two microliters without a cuvette is performed on the NanoDrop spectrophotometer. The Typhoon Trio Imager measure and image radioactive signals using from gels or membranes using phosphor screens and fluorescence from gels, membranes, TLC plates, and microtiter plates. Other instruments include Beckman Biomek NX and FX pipetting robots, and Berthold NightOWL. The NightOwl is available for low-light imaging of luminescence or fluorescence in plants or animals.
