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(I) Sample Requirements

A. Sanger Sequencing

 Individual Samples (<24 samples)

    • Use a 1.5 mL microfuge tube
    • Provide at least 10 ul of sample
    • Centrifuge your sample before dropping it off at DSF
    • Write your order number legibly on the cap like this:

                                                

    • Write your sample number legibly on the cap like this:

                 

96 Well Plate (>24 samples)

 

    • Use a 96 well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec plates)
    • Provide at least 10 ul of sample
    • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
    • Centrifuge your plate before dropping it off at DSF
    • Write your order number legibly on the foil
    • Put your samples in order vertically, starting with well A1, as shown below:


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