Sample Requirements

Sanger Sequencing

A.  Individual Samples (<24 samples)

• • Use a 1.5 mL microfuge tube
  • Provide at least 10 ul of sample
  • Centrifuge your sample before dropping it off at DSF
  • Write your order number legibly on the cap like this:

• • Write your sample number legibly on the cap like this:

B.  96 Well Plate (>24 samples)

• • Use a 96 well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec plates)
  • Provide at least 10 ul of sample
  • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
  • Centrifuge your plate before dropping it off at DSF
  • Write your order number legibly on the foil
  • Put your samples in order vertically, starting with well A1, as shown below:

B.  PCR Cleanup

 A.  Individual Samples (<24 samples)

• • Use a 1.5 mL microfuge tube
  • Provide at least 20 ul of unpurified PCR product
  • Provide at least 3 ul of 10 uM primer per sample (please pool like primer in same tube)
  • Centrifuge your sample before dropping it off at DSF
  • Write your order number legibly on the cap like this:

• • Write your sample number legibly on the cap like this:

•       
  • Write your primer legibly on the cap like this: