Submission Checklists:
Sanger DNA Sequencing Submission Checklist.pdf
PCR Cleanup Submission Checklist.pdf
We reserve the right to delay or refuse any order with samples that do not meet the requirements below!
Sanger Sequencing
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 10 uL of sample
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap like this:
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 10 uL of sample
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the foil
- Put your samples in order vertically, starting with well A1, as shown below:
PCR Cleanup
A. Individual Samples (<24 samples)
- Use a 1.5 mL microfuge tube
- Provide at least 20 uL of unpurified PCR reaction
- Centrifuge your sample before dropping it off at DSF
- Write your order number and sample NUMBER legibly on the cap like this:
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap like this:
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
B. 96 Well Plate (>24 samples)
- Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
- Provide at least 20 uL of unpurified PCR reaction
- Seal your plate with adhesive foil (DSF uses VWR 60941-074)
- Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
- Write your order number legibly on the foil
- Put your samples in order vertically, starting with well A1, as shown below:
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
- Primer should be provided at 10 uM concentration
- Write the order number AND primer name legibly on the cap like this:
- Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
What NOT to do!
PLEASE NOTE:
Our goal is quick processing of samples for a quick turnaround time. Thus, please do NOT do any of the following:
- Do NOT pool like samples in one tube! # of sample tubes must match # of requested sequencing reactions (1:1)
- Do NOT write your sample NAME on the lid of the microfuge tube
- Do NOT submit primer separately for Sanger sequencing orders (okay for PCR Cleanup only)
- Do NOT use 0.5 mL microfuge tubes for samples or primers
- Do NOT submit non-centrifuged samples. If there is at least 4 uL at bottom, we will use as is.
- Do NOT submit orders of >48 samples in individual tubes
- Do NOT use standard aluminum foil to cover your 96 well plates
Thank you for your cooperation!
